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University of Cincinnati / Institutional Biosafety Commitee IBC Protocol - Main Form This form consists of 8 sections. You can save entered data and resume it later, if necessary. Depending on your research items, other forms will need to be completed.
______ ______ Biosafety Office Use ONLY IBC Number: ______ Last Submission: ______ Initial APPROVAL DATE : ______ Last Update Approval: ______
The title should be something broad so it covers current and/or future projects
Section I. A: Principal Investigator
First Name: Last Name: Job Title: E-Mail Address: Department: UC Mail Code: Office Phone: Cell Phone:
First & Last Name:
Job Title: Department:
UC Mail Code:
Office Phone:
Cell Phone:
Lab Phone:
E-Mail Address:
First & Last Name Job Title Email Phone Number
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Section I. D: Experiment Location(s)
BSC a.k.a tissue culture hood Building Name Room Number Type/Function of Location (e.g. main lab, open bench lab, tissue culture room, cold room, storage room, freezer room)
BSC present?
Shared Space?
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BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UC Bioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UC Bioscience
BAWALT CARE Crosley T CVC ERC French E Hoxworth Kettering MSB RC A RC B RC C RC D RC E RC F RC G RC J RC K Rhodes Rieveschl SRU VONTZ VPC OTHER UCBioscience
Type/Function of Location 1
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 2
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 3
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 4
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
Type/Function of Location 5
e.g. Main Lab, open lab bench, tissue culture room, freezer room, cold room, microscopy room
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Are non-UC Facilities used in this protocol? If YES , specify facility (institution/location) and function.
Are non-UC Facilities used in this protocol?
YES NO
Specify facility (institution/location) used and its function.
Section II. A: Research Items
Check All that apply:
Recombinant or Synthetic Nucleic Acids, including Viral Vectors (Ref: NIH Guidelines Sections III - A to E ) Microbial/Infectious Agents (e.g., Bacteria, Viruses, Fungi, Protozoa, Transducing Proteins) NO viral vector Human/Non-Human Primate Derived Materials (including established cell lines) Biohazardous Materials used in Live Animals Biohazardous Materials used with Radioactive Materials (RAM) or Ionizing Radiation Biological Toxins Within the Select Agents Permissible Amounts (Ref: Select Agent Program - toxins ) The IBC purview only covers toxins within the Select Agent list. If within the exempt limits, info on toxins do not need to be included in this protocol, but Form F must be completed and forwarded to the IBC. Biological Toxins Above the Select Agents Permissible Amounts (Ref: Select Agent Program - toxins )
Section II. B: Research Description - Abstract Provide a brief summary of the purpose of your research project(s) describing how the materials indicated above will be used. DO NOT include details about the research experiments and safety precautions; these will be asked in further sections. If you have more than one project you may separate info by project 1, project 2 etc.
Indicate the highest biosafety level adopted in your lab.
* BSL2 plus corresponds to the use of a BSL2 facility adopting BSL3 practices, including the use of fit-tested respirators (e.g. N-95) for aerosol generating activities that are performed outside of a Biosafety Cabinet.
Indicate the highest biosafety level adopted in your lab.
BSL1 BSL2 BSL2 plus* BSL3
BSL2 plus corresponds to the use of a BSL2 facility adopting BSL3 practices, including the use of fit-tested respirators (e.g. N-95) for aerosol generating activities that are performed outside of a Biosafety Cabinet.
Indicate whether your research will involve any of the following:
Renders a useful vaccine ineffective Enhances pathogen virulence and/or increases pathogen transmissibility Widens the pathogen's host range Environmental stabilization of pathogens Deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally and such acquisition could compromise the ability to control disease agents in humans, veterinary medicine, or agriculture (Ref: NIH Guidelines Section III- A-1 ) Cloning of toxin molecules with LD50 of less than 100 nanograms per kilogram body weight (Ref: NIH Guidelines Section III - B and Univ. Florida LD50 table ) Regulated biological toxins (exceeding the exempt amounts) (Ref: Select Agents Program - toxins )
Renders a useful vaccine ineffective
YES NO
Enhances pathogen virulence and/or increases pathogen transmissibility
YES NO
Widens the pathogen's host range
YES NO
Environmental stabilization of pathogens
YES NO
Deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally (Ref: NIH Guidelines Section III- A-1)
YES NO
Cloning of toxin molecules with LD50 of less than 100 nanograms per kilogram body weight (Ref: NIH Guidelines Section III - B and Univ. Florida LD50 table)
YES NO
Regulated biological toxins (exceeding the exempt amounts) (Ref: Select Agents Program - toxins)
YES NO
Do the planned experiments involve the generation or use of more than 10 L of culture in a s ingle vessel ?
If YES , explain the culture procedures, including location, types of equipment used, special precautions to handle large volumes:
Do the planned experiments involve the generation or use of more than 10 liters of culture in a single vessel?
YES
NO
If YES, explain the culture procedures, including location, types of equipment used, special precautions to handle large volumes:
Section III. A: Aerosol Producing Lab Equipment
For each aerosol producing equipment checked, describe your mitigation plan to prevent exposure (e.g. containment equipment, procedures). Go HERE for the Biosafety eManual on Aerosol Exposure Control.
N/A
Centrifuge
Cell Sorter
Ultracentrifuge
Vortex
Pipet
Homogenizer
Tissue Grinder
Blender
Sonicator
Other
Section III. B - Engineering Controls & Safety Equipment
Engineering Controls eliminate or reduce exposure to a hazard through the use or substitution of engineered machinery or equipment.
Select the engineering control that is used to handle biohazardous materials. If Fume Hood is only used for hazard chemicals DO NOT check the box for that.
TRAINING REQUIREMENT: Individuals using a BSC must complete the Biosafety Cabinets training
Additional Information?
Yes No
Do not include PPE used at the LAMS facilities. PPE information can be found HERE
Yes No
PPE (gloves, lab coat, body suit, f mask, shoes)
PPE (dgloves, fr lab coat, saf goggles, face shield, shoe cov)
Primary container(s) with infectious or potentially infectious materials is transferred between labs inside a specially designated leak-proof secondary container labeled with the universal biohazard symbol. Secondary container also should have enough absorbent material to totally contain a spill should the primary container fail. If materials are transported in public streets, a tertiary container (e.g. cardboard box) is used. When materials are shipped out-of-UC Campus, the Biosafety Office is contacted for information about shipping requirements.
I will follow the above method. I have a different method (describe below).
Yes No
For proper disinfection, disinfectants must remain in contact with the material or surface to ensure proper disinfection. Contact time will vary depending on the product and organism targeted; read the label of the brand you choose.
Check ALL that apply
Additional Information? IMPORTANT INFORMATION:
* Alcohol based solutions evaporate too quickly (especially if used inside a BSC). Surface might need to be saturated to achieve proper contact time (around 1 to 2 min ).
** The contact time for Sodium Hypochlorite is normally 5 min. Sodium Hypochlorite is corrosive and to prevent damage to metal surfaces, after sufficient contact time, rinse surfaces with sterile water or 70% ethanol. SEE how to prepare a 10% bleach solution
Yes No
Disinfectant (ethanol, clorox, Super sanicloth)
disinfectants (bleach, dispatch, sporklens)
Biohazardous waste is managed by the Environmental Health and Safety (EH&S) office. The procedures for disposal of biohazardous materials are outlined in their Advisory 10.2
I will follow the EH&S procedures (Advisory 10.2).
Yes No
It is important that all authorized personnel be prepared to respond to a spill involving infectious or potentially infectious materials. The Biosafety Office developed procedures for spills involving biohazardous materials.
I will follow the Biosafety Office procedures for spills involving biohazardous materials.
Yes No
List all biohazardous materials which are NOT actively being used and which will only be maintained in storage for potential future use (Note: IBC approval will be needed to actively use these materials in the future). Biohazardous Material Storage Location Storage Description
(e.g. freezer, liquid nitrogen)
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